LatticeMLS

Bench

Dilution Bench

Prepare a 1:5 dilution and follow the measured result through correction.

Prepare the dilution

Worked example

1:5 preparation and correction

Preparation
100 µL specimen + 400 µL diluent = 500 µL
Measurement
4180.0 ÷ 5 = 836.0 U/L
Correction
836.0 × 5 = 4180.0 U/L
One specimen part with four diluent parts makes a 1:5 dilution; one specimen part with five diluent parts makes six total parts.
A 1:5 preparation has five total parts. Adding five diluent parts to one specimen part makes six total parts and requires factor 6.

Order review

Dilution preparation

The initial CK result is above the measuring interval. Prepare the ordered dilution, measure it, and apply the factor that matches the mixture.

The initial CK result is above the measuring interval. The dilution order is ready.

Read the full explanation in Dilution Math.1-3The tube drawing is based on the 6 mL BD Vacutainer SST II Advance tube.4

References

  1. Bishop ML, Fody EP, Van Siclen C, Mistler JM, Moy M. Clinical Chemistry: Principles, Techniques, and Correlations. 9th ed. Jones & Bartlett Learning; 2023.
  2. College of American Pathologists. Analytical Measurement Range. Accessed August 25, 2026. Applies to CAP-accredited laboratories.
  3. Clinical and Laboratory Standards Institute. Establishing and Verifying an Extended Measuring Interval Through Specimen Dilution and Spiking. 1st ed. CLSI guideline EP34. Clinical and Laboratory Standards Institute; 2018. Reaffirmed March 2023. Accessed August 25, 2026.
  4. BD. BD Vacutainer SST II Advance 6 mL 16 × 100 mm. Product 366444. Accessed August 25, 2026.