Dilution station

Dilute a CK result that is above the analyzer's measuring range, measure the dilution and calculate the patient result. Serial dilutions, titer racks and worksheet reviews use the same bench.

What to know first: Dilution Math
Laboratory Mathematics and Diagnostic Performance
How long it takes: About 5 minutes for a single dilution and about 10 for a series.
What's free: The first CK order, a 1:5 dilution, opens without an account. A free account opens every case.

Dilute the first CK orderWorking at the bench needs JavaScript.

Dilution station

When CK is over the analyzer's 50 to 1000 U/L range, dilute it, rerun, and multiply the reading by the dilution factor. JavaScript is required to work through the case.

Parts: A 1:5 dilution is 1 part specimen + 4 parts diluent = 5 total parts
Specimen part: Final volume ÷ total parts
Patient result: Measured diluted result × total parts

One specimen part with four diluent parts makes a 1:5 dilution. One specimen part with five diluent parts makes six total parts. — One part specimen plus five parts diluent is six parts, and that needs factor 6.

Read the dilution math lesson

Serial dilutions

A 100 mg/L solution has 100 mg in every liter. Diluent adds volume and no analyte. An aliquot is the measured portion you move.

In a twofold series each mixed tube feeds the next: 100 → 50 → 25 → 12.5 mg/L. Total factors are 2, 4, and 8. Pulling an aliquot off a mixed tube leaves its concentration where it was.

JavaScript is required for the serial dilution controls. Read serial dilution explanations.