LatticeMLS
Menu

Module UBF-11 · Version 1.0

Pleural and Peritoneal Fluid Evaluation

A pleural fluid case walks through container selection, gross appearance, cell count and differential, and Light's criteria calculated against paired serum results, then draws the boundary between pleural and peritoneal frameworks and between validated and unvalidated body-fluid chemistry.

Your next section

Start section 1

Read without an account. Sign in to save.

Who this module is for

Clinical laboratory scientists and students performing or reviewing pleural and peritoneal fluid testing

Learning objectives

  • Select containers and aliquots for cell count, chemistry, microbiology, and cytology on a serous fluid request
  • Perform or assess a total nucleated cell count and differential and calculate the absolute neutrophil count
  • Use paired fluid and serum data with current validated criteria without extending them beyond the fluid type and matrix they were built for

How completion works

Mark every required section done and answer every knowledge check in those sections correctly. The final save completes the module automatically.

Sources

14 sources

  1. 1. Clinical and Laboratory Standards Institute. H56-A: Body Fluid Analysis for Cellular Composition; Approved Guideline, 1st ed., 2006. https://clsi.org/shop/standards/h56/

    Source note · consensus standard

  2. 2. Clinical and Laboratory Standards Institute. C49: Analysis of Body Fluids in Clinical Chemistry, 2nd ed., 2018. https://clsi.org/shop/standards/c49/

    Source note · consensus standard

  3. 3. Centers for Medicare & Medicaid Services. 42 CFR 493.1251, Standard: Procedure manual, current eCFR text. https://www.ecfr.gov/current/title-42/chapter-IV/subchapter-G/part-493/subpart-K/section-493.1251

    Source note · federal guidance

  4. 4. Centers for Medicare & Medicaid Services. 42 CFR 493.1253, Standard: Establishment and verification of performance specifications, current eCFR text. https://www.ecfr.gov/current/title-42/chapter-IV/subchapter-G/part-493/subpart-K/section-493.1253

    Source note · federal guidance

  5. 5. Centers for Medicare & Medicaid Services. 42 CFR 493.1291, Standard: Test report, current eCFR text. https://www.ecfr.gov/current/title-42/chapter-IV/subchapter-G/part-493/subpart-K/section-493.1291

    Source note · federal guidance

  6. 6. College of American Pathologists. All Common Checklist, COM.40620 Body Fluid Analysis (Phase II). CAP Accreditation Program, 12.09.2025 edition.

    Source note · accreditation standard

  7. 7. College of American Pathologists. Hematology, Coagulation, and Clinical Microscopy Checklist, HEM.35357 Body Fluid Analysis Procedure (Phase II). CAP Accreditation Program, 12.09.2025 edition.

    Source note · accreditation standard

  8. 8. College of American Pathologists. Hematology, Coagulation, and Clinical Microscopy Checklist, HEM.35528 Body Fluid Cell Differentials (Phase I). CAP Accreditation Program, 12.09.2025 edition.

    Source note · accreditation standard

  9. 9. Light RW, Macgregor MI, Luchsinger PC, Ball WC Jr. Pleural effusions: the diagnostic separation of transudates and exudates. Annals of Internal Medicine. 1972;77(4):507-513. https://doi.org/10.7326/0003-4819-77-4-507

    Source note · peer-reviewed literature

  10. 10. Roberts ME, Rahman NM, Maskell NA, et al. British Thoracic Society Guideline for pleural disease. Thorax. 2023;78(Suppl 3):s1-s42. https://thorax.bmj.com/content/78/Suppl_3/s1

    Source note · professional society guidance

  11. 11. Rahman NM, Mishra EK, Davies HE, et al. Clinically important factors influencing the diagnostic measurement of pleural fluid pH and glucose. American Journal of Respiratory and Critical Care Medicine. 2008;178(5):483-490. https://pmc.ncbi.nlm.nih.gov/articles/PMC2643213/

    Source note · peer-reviewed literature

  12. 12. Biggins SW, Angeli P, Garcia-Tsao G, et al. Diagnosis, evaluation, and management of ascites, spontaneous bacterial peritonitis and hepatorenal syndrome: 2021 practice guidance by the American Association for the Study of Liver Diseases. Hepatology. 2021;74(2):1014-1048. https://doi.org/10.1002/hep.31884

    Source note · professional society guidance

  13. 13. Kaltenbach M. Why do we use antibiotics for SBP prophylaxis? American Association for the Study of Liver Diseases, 2021. https://www.aasld.org/liver-fellow-network/core-series/why-series/why-do-we-use-antibiotics-sbp-prophylaxis

    Source note · professional society guidance

  14. 14. Local laboratory controlled copy. Exact analyzer and reagent-family instructions for use, application sheet, software release notes, and service bulletins. No universal URL; verify the local controlled revision before production.

    Source note · manufacturer labeling