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Module UBF-14 · Version 1.0

Semen Analysis: From Collection to Result Interpretation

Work through a basic semen examination from collection history through microscopic classification. Confirm abstinence, collection completeness, and timing; calculate concentration and total sperm number from raw chamber counts; classify motility using current WHO terminology; and decide what a single result set can and cannot say about fertility.

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Who this module is for

Clinical laboratory scientists and students with prior microscopy, cell counting, and specimen handling experience who perform or review basic semen examinations.

Learning objectives

  • Explain the abstinence, complete collection, container, transport, temperature, and timing requirements a semen specimen must meet before results can be trusted.
  • Measure or evaluate volume, liquefaction, viscosity, concentration, total sperm number, motility, vitality, and morphology using WHO sixth-edition procedures and stated reference limits.
  • Distinguish descriptive laboratory findings from a stand-alone fertility determination and write a defensible report limitation.

How completion works

Mark every required section done and answer every knowledge check in those sections correctly. The final save completes the module automatically.

Sources

14 sources

  1. 1. World Health Organization. WHO Laboratory Manual for the Examination and Processing of Human Semen. 6th ed. Geneva: WHO Press; 2021.

    Source note · consensus standard

  2. 2. Chung E, Atmoko W, Saleh R, Shah R, Agarwal A. Sixth edition of the World Health Organization laboratory manual of semen analysis: updates and essential take away for busy clinicians. Arab J Urol. 2023;22(2):71-74.

    Source note · peer-reviewed literature

  3. 3. Electronic Code of Federal Regulations. 42 CFR 493.1291, Standard: Test report. Centers for Medicare & Medicaid Services.

    Source note · federal regulation

  4. 4. Centers for Disease Control and Prevention. Test Complexities. Clinical Laboratory Improvement Amendments (CLIA).

    Source note · federal regulation

  5. 5. American Urological Association. Vasectomy: AUA Guideline.

    Source note · professional society guidance

  6. 6. College of American Pathologists. Hematology, Coagulation, and Clinical Microscopy Checklist, HEM.35699 Specimen Collection/Handling (Phase I). CAP Accreditation Program, 12.09.2025 edition.

    Source note · accreditation standard

  7. 7. College of American Pathologists. Hematology, Coagulation, and Clinical Microscopy Checklist, HEM.35737 Specimen Handling - Pre-analytic (Phase I). CAP Accreditation Program, 12.09.2025 edition.

    Source note · accreditation standard

  8. 8. College of American Pathologists. Hematology, Coagulation, and Clinical Microscopy Checklist, HEM.35718 Liquefaction (Phase I). CAP Accreditation Program, 12.09.2025 edition.

    Source note · accreditation standard

  9. 9. College of American Pathologists. Hematology, Coagulation, and Clinical Microscopy Checklist, HEM.35756 Specimen Characteristics - Analytic (Phase I). CAP Accreditation Program, 12.09.2025 edition.

    Source note · accreditation standard

  10. 10. College of American Pathologists. Hematology, Coagulation, and Clinical Microscopy Checklist, HEM.35775 Motility/Progression Evaluation (Phase II). CAP Accreditation Program, 12.09.2025 edition.

    Source note · accreditation standard

  11. 11. College of American Pathologists. Hematology, Coagulation, and Clinical Microscopy Checklist, HEM.35822 Viability Testing Criteria (Phase I). CAP Accreditation Program, 12.09.2025 edition.

    Source note · accreditation standard

  12. 12. College of American Pathologists. Hematology, Coagulation, and Clinical Microscopy Checklist, HEM.35832 Sperm Morphology Classification (Phase I). CAP Accreditation Program, 12.09.2025 edition.

    Source note · accreditation standard

  13. 13. College of American Pathologists. Hematology, Coagulation, and Clinical Microscopy Checklist, HEM.35893 Leukocyte Confirmation Techniques (Phase I). CAP Accreditation Program, 12.09.2025 edition.

    Source note · accreditation standard

  14. 14. College of American Pathologists. Hematology, Coagulation, and Clinical Microscopy Checklist, HEM.35661 Azoospermic Specimen Result Reporting (Phase I). CAP Accreditation Program, 12.09.2025 edition.

    Source note · accreditation standard