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Module GLP-07 · Version 1.0

Centrifuge Operation, Balance, and Safety

A working guide to relative centrifugal force, rotor selection, balanced loading, and safe response to centrifuge defects and spills, built around a serum-tube run.

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Who this module is for

Laboratory professionals and students who operate or evaluate clinical centrifuges

Learning objectives

  • Distinguish RPM from relative centrifugal force and calculate or verify RCF
  • Balance loads by mass and position and select compatible rotors, tubes, and adapters
  • Recognize rotor damage, tube failure, aerosol risk, incomplete separation, and maintenance needs, and choose a defensible response

How completion works

Mark every required section done and answer every knowledge check in those sections correctly. The final save completes the module automatically.

Sources

10 sources

  1. 1. Eppendorf. Centrifuge 5415 C Instruction Manual. Manufacturer operating manual. https://www.nist.gov/system/files/documents/ncnr/Centrifuge_Eppendorf_5415C.pdf

    Source note · manufacturer labeling

  2. 2. Thermo Fisher Scientific. Centrifuge Best Practices. Manufacturer operating guidance. https://www.thermofisher.com/us/en/home/life-science/lab-equipment/lab-equipment-learning-center/centrifuges/centrifuge-best-practices.html

    Source note · manufacturer labeling

  3. 3. Becton, Dickinson and Company. BD Vacutainer Evacuated Blood Collection System, package insert. Manufacturer labeling. https://www.bd.com/documents/alerts/Vacutainer%20Recall%20-%20Updated%20Package%20Insert.pdf

    Source note · manufacturer labeling

  4. 4. Kitchen S, et al. International Council for Standardization in Haematology recommendations for processing of blood samples for coagulation testing. International Journal of Laboratory Hematology. 2022;44:219-229. https://doi.org/10.1111/ijlh.13702

    Source note · consensus standard

  5. 5. Centers for Disease Control and Prevention. Provider Performed Microscopy Procedures: A Focus on Quality Practices, 2025. Federal educational guidance. https://www.cdc.gov/lab-quality/media/pdfs/2025/07/PPMP_2025_Final-508c.pdf

    Source note · federal guidance

  6. 6. Centers for Medicare & Medicaid Services. State Operations Manual, Appendix C: Survey Procedures and Interpretive Guidelines for Laboratories and Laboratory Services, Rev. 236, issued 2026-01-23. https://www.cms.gov/Regulations-and-Guidance/Guidance/Manuals/downloads/som107ap_c_lab.pdf

    Source note · federal guidance

  7. 7. 42 CFR §493.1254, Standard: Maintenance and function checks. Federal regulation, United States. https://www.ecfr.gov/current/title-42/chapter-IV/subchapter-G/part-493/subpart-K/section-493.1254

    Source note · federal regulation

  8. 8. Clinical and Laboratory Standards Institute. QMS23: General Laboratory Equipment Performance Qualification, Use, and Maintenance, current edition catalog record. https://clsi.org/standards/products/quality-management-systems/documents/qms23/

    Source note · consensus standard

  9. 9. Centers for Disease Control and Prevention and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories, 6th ed. https://www.cdc.gov/labs/bmbl/index.html

    Source note · federal guidance

  10. 10. Occupational Safety and Health Administration. 29 CFR 1910.1030, Bloodborne pathogens. https://www.osha.gov/laws-regs/regulations/standardnumber/1910/1910.1030

    Source note · federal regulation