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Section 5 of 6 · Open sections

Required section · Section 5 of 6

Four specimens, four decisions

You are covering the blood gas bench. Four specimens arrive in sequence, each with one defect. Under a supplied local policy, decide whether to accept and analyze as drawn, recollect before analysis, or report with an explicit caveat describing the affected direction of change. This decision requires naming the defect, predicting which measured values it pushes and in which direction, and choosing the bounded action the policy allows, not just recognizing that something is wrong.

Specimen 1 arrives with a visible 0.3 mL air bubble still mixed into the blood; it was never expelled. Specimen 2 was drawn correctly but sat on the counter for 45 minutes before anyone noticed it, outside the laboratory's validated syringe/specimen/analyzer window. Specimen 3 was drawn into a syringe with excess liquid heparin rather than the standard dry, electrolyte-balanced syringe, leaving visibly diluted blood. Specimen 4 was drawn from an arterial line without discarding the flush volume first, so it is contaminated with heparinized saline.

For each specimen, identify the defect, state its expected direction where known (or that it is unpredictable), and apply the laboratory's clear local recollection rule: recollect when a specimen defect makes the result unsuitable for its intended use or when local acceptance criteria are not met. A caveated report is used only where local policy explicitly permits it.

Telling a measured PCO2 from a calculated bicarbonate is what makes this distinction usable; conflating the two leads to misjudging which number to distrust. Name the defect and its direction before you choose accept, recollect, or caveat, because the policy rule depends on that reasoning, not on the result looking abnormal.

Ordering exercise

Place these arterial blood gas collection steps in the correct sequence to minimize preanalytical error.

  1. 1. Mix by gentle rolling

    Roll the syringe to distribute heparin through the blood and reduce the risk of microclot formation.

  2. 2. Transport promptly for analysis

    Analyze the plastic-syringe specimen within the laboratory's locally validated syringe/specimen/analyzer window using its named local handling workflow; do not apply that workflow universally.

  3. 3. Discard adequate line-flush volume

    When drawing from an indwelling arterial line, discard enough flush/dead-space volume first so the specimen is not diluted with flush solution.

  4. 4. Expel any visible air bubble

    Promptly expel a visible bubble from the capped syringe before mixing, rather than mixing it into the specimen.

  5. 5. Draw specimen into dry heparin syringe

    Collect the specimen into a dry, electrolyte-balanced heparin syringe to avoid the dilution risk of liquid heparin.

Knowledge checks

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Knowledge check 1

Specimen 2 sat for 45 minutes before analysis, outside the laboratory's locally validated syringe/specimen/analyzer window, with no other defect. What is the defensible action?

Choose one option.

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