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Module GLP-08 · Version 1.0

Pipette Technique, Verification, and Maintenance

Manual pipetting technique creates systematic or random volume error; a laboratory verifies pipette performance with gravimetric data. You will select an air-displacement or positive-displacement pipette for a given liquid, follow the technique variables that most affect delivery, calculate bias and imprecision from a gravimetric data set, and diagnose a replicate error pattern using supplied criteria.

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Who this module is for

Clinical laboratory scientists, medical laboratory technicians, and trainees who perform manual pipetting for testing, dilutions, or quality control preparation.

Learning objectives

  • Distinguish pipette types and select the correct volume range and tip for a given liquid and task
  • Perform prewetting, aspiration, dispensing, angle, immersion, speed, and blow-out correctly for forward and reverse pipetting
  • Calculate delivered volume, bias, and imprecision from gravimetric data and use supplied criteria to reach a pass or fail decision

How completion works

Mark every required section done and answer every knowledge check in those sections correctly. The final save completes the module automatically.

Sources

12 sources

  1. 1. International Organization for Standardization. ISO 8655-1:2022, Piston-operated volumetric apparatus, Part 1: Terminology, general requirements and user recommendations. 2nd ed., 2022. https://www.iso.org/standard/68796.html

    Source note · consensus standard

  2. 2. International Organization for Standardization. ISO 8655-2:2022, Piston-operated volumetric apparatus, Part 2: Pipettes. 2nd ed., 2022. https://www.iso.org/standard/68797.html

    Source note · consensus standard

  3. 3. International Organization for Standardization. ISO 8655-6:2022, Piston-operated volumetric apparatus, Part 6: Gravimetric methods for the determination of measurement error. 2nd ed., 2022. https://www.iso.org/standard/68799.html

    Source note · consensus standard

  4. 4. Clinical and Laboratory Standards Institute. QMS23, General Laboratory Equipment Performance Qualification, Use, and Maintenance. 2nd ed., 2019. https://clsi.org/shop/standards/qms23/

    Source note · consensus standard

  5. 5. Centers for Medicare & Medicaid Services. 42 CFR 493.1252, Standard: Test systems, equipment, instruments, reagents, materials, and supplies. Current eCFR text mirrored by Cornell Legal Information Institute. https://www.law.cornell.edu/cfr/text/42/493.1252

    Source note · federal regulation

  6. 6. Centers for Medicare & Medicaid Services. 42 CFR 493.1254, Standard: Maintenance and function checks. Current eCFR text mirrored by Cornell Legal Information Institute. https://www.law.cornell.edu/cfr/text/42/493.1254

    Source note · federal regulation

  7. 7. College of American Pathologists. All Common Checklist, COM.30600 Maintenance/Function Checks (Phase II). CAP Accreditation Program, 12.09.2025 edition.

    Source note · accreditation standard

  8. 8. Eppendorf SE. Eppendorf Reference 2 Operating Manual. https://www.eppendorf.com/product-media/doc/en/176179/Eppendorf_Liquid-Handling_Operating-manual_Reference-2_Eppendorf-Reference-2.pdf

    Source note · manufacturer labeling

  9. 9. METTLER TOLEDO. Pipette Choice and Maintenance. Rainin white paper. https://www.mt.com/dam/RAININ/PDFs/White_Papers/WP_choosing-and-maintaining-your-pipes.pdf

    Source note · manufacturer labeling

  10. 10. Guan XL, Chang DPS, Mok ZX, Lee B. Assessing variations in manual pipetting: An under-investigated requirement of good laboratory practice. Journal of Mass Spectrometry and Advances in the Clinical Lab. 2023;30:25-29. doi:10.1016/j.jmsacl.2023.09.001. https://pmc.ncbi.nlm.nih.gov/articles/PMC10569977/

    Source note · peer-reviewed literature

  11. 11. Batista E, Almeida N, Filipe E. A study of factors that influence micropipette calibrations. NCSLI Measure: The Journal of Measurement Science. 2015;10(1):60-66. doi:10.1080/19315775.2015.11721717. https://www.tandfonline.com/doi/abs/10.1080/19315775.2015.11721717

    Source note · peer-reviewed literature

  12. 12. Mervin SM, et al. The pipetting Olympics: Propagating proper pipetting a priori in clinical LC-MS/MS analysis. Journal of Applied Laboratory Medicine. 2023. https://pmc.ncbi.nlm.nih.gov/articles/PMC10368908/

    Source note · peer-reviewed literature