Module GLP-24 · Version 1.0
Biosafety Cabinets, Fume Hoods, and Infectious Materials
Match a containment device to its hazard, work in a Class II biosafety cabinet without disrupting containment, and respond to a suspected aerosol release from a broken centrifuge cup.
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Who this module is for
Clinical laboratory technologists and students working toward independent bench competency in microbiology and general laboratory safety.
Learning objectives
- Match device airflow and protection type to a biological, chemical, product, or radiological hazard
- Set up and work in a Class II biosafety cabinet without disrupting containment
- Recognize aerosol-generating procedures, certification needs, spills, decontamination, and transport controls
How completion works
Mark every required section done and answer every knowledge check in those sections correctly. The final save completes the module automatically.
Sources
12 sources
1. CDC/NIH. Biosafety in Microbiological and Biomedical Laboratories (BMBL), 6th edition.
Source note · federal guidance
2. NSF International. Biosafety Cabinet Certification and Biosafety Cabinet Field Certifier Accreditation program pages; NSF/ANSI 49 field-certification framework.
Source note · consensus standard
3. NSF International. NSF/ANSI 49 Annex informative material on BSC installation, sash operation, and grille clearance.
Source note · consensus standard
4. CDC REACH. Fundamentals of Working Safely in a Biological Safety Cabinet (BSC), airflow training transcript and safe-use checklist.
Source note · federal guidance
5. OSHA. 29 CFR 1910.1030, Bloodborne Pathogens, including HIV/HBV research-laboratory provisions at (e)(2)(iii) and decontamination requirements at (d)(4).
Source note · federal regulation
6. OSHA. 29 CFR 1910.1450, Occupational Exposure to Hazardous Chemicals in Laboratories, and non-mandatory Appendix A.
Source note · federal regulation
7. OSHA. 29 CFR 1910.1200, Hazard Communication, including the 2024 final rule and enforcement implementation guidance.
Source note · federal regulation
8. NIH Office of Science Policy. NIH Guidelines for Research Involving Recombinant or Synthetic Nucleic Acid Molecules, Appendix B, Risk Groups.
Source note · federal guidance
9. CLSI. M29-A4, Protection of Laboratory Workers From Occupationally Acquired Infections, approved guideline, 4th edition.
Source note · consensus standard
10. College of American Pathologists. Laboratory Accreditation Program, Microbiology Checklist, biological safety cabinet certification requirement.
Source note · accreditation standard
11. US DOT/PHMSA. 49 CFR Part 173, Subpart E, sections 173.134, 173.196, 173.199; PHMSA guidance, Transporting Infectious Substances Safely.
Source note · federal regulation
12. NIH Office of Research Services, Division of Occupational Health and Safety. BSL-2 Laboratory Biosafety Manual, centrifuge spill and settling-time guidance.
Source note · federal guidance