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Module GLP-26 · Version 1.0

Compressed Gases, Cryogens, Dry Ice, and Liquid Nitrogen

Covers safe handling of compressed-gas cylinders and cryogens in the laboratory: cylinder inspection, securement, and segregation; liquid nitrogen (LN2) and dry ice hazards from extreme cold, expansion, and oxygen displacement; personal protective equipment for cryogen transfer; and emergency response to a low-oxygen alarm or a suspect sealed cryovial.

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Who this module is for

Laboratory staff and technologists who inspect, store, transport, or use compressed-gas cylinders, dry ice, or liquid nitrogen as part of routine bench or specimen-handling work.

Learning objectives

  • Identify pressure, projectile, asphyxiation, frostbite, embrittlement, and overpressurization hazards from compressed gases and cryogens
  • Inspect, secure, move, connect, and segregate gas cylinders safely
  • Handle dry ice and liquid nitrogen with correct ventilation, compatible vessels, face/skin protection, and nonsealed containers

How completion works

Mark every required section done and answer every knowledge check in those sections correctly. The final save completes the module automatically.

Sources

15 sources

  1. 1. OSHA. 29 CFR 1910.101, Compressed gases (general requirements).

    Source note · federal regulation

  2. 2. OSHA. 29 CFR 1910.253, Oxygen-fuel gas welding and cutting.

    Source note · federal regulation

  3. 3. OSHA. 29 CFR 1910.146, Permit-required confined spaces.

    Source note · federal regulation

  4. 4. OSHA. 29 CFR 1910.1200 Appendix C, Allocation of Label Elements (Hazard Communication Standard).

    Source note · federal regulation

  5. 5. OSHA. Laboratory Safety: Cryogens and Dry Ice (Quick Facts), OSHA 3408, Rev. 10/2011.

    Source note · federal guidance

  6. 6. OSHA. Hazard Information Bulletin HIB 96-06-05, Potential Carbon Dioxide (CO2) Asphyxiation Hazard When Filling Stationary Low Pressure CO2 Supply Systems.

    Source note · federal guidance

  7. 7. OSHA. Chemical Sampling Information: Carbon Dioxide (PEL/REL/IDLH reference page).

    Source note · federal guidance

  8. 8. OSHA. News release, Investigation into 41-year-old driver's death finds two delivery services companies failed to protect workers transporting dry ice in Alabama, and linked accident-investigation detail records.

    Source note · federal regulation

  9. 9. Compressed Gas Association. CGA C-9, Standard Color Marking of Compressed Gas Containers for Medical Use (current edition).

    Source note · consensus standard

  10. 10. College of American Pathologists. Laboratory General Checklist, GEN.76800 Gas Cylinder Storage (Phase II). CAP Accreditation Program, 12.09.2025 edition. College of American Pathologists. Laboratory General Checklist, GEN.76900 Flammable Gas Cylinders (Phase II). CAP Accreditation Program, 12.09.2025 edition.

    Source note · accreditation standard

  11. 11. National Fire Protection Association. NFPA 55, Compressed Gases and Cryogenic Fluids Code, 2023 edition.

    Source note · consensus standard

  12. 12. US DOT/PHMSA. 49 CFR 173.217, Carbon dioxide, solid (dry ice).

    Source note · federal regulation

  13. 13. US DOT/PHMSA. 49 CFR 173.199, Category B infectious substances.

    Source note · federal regulation

  14. 14. American Heart Association / American Red Cross. 2024 Guidelines for First Aid (cold-injury/frostbite rewarming section).

    Source note · professional society guidance

  15. 15. Stanford University Environmental Health & Safety. Cryogenic Vial Safety.

    Source note · laboratory reference