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Module UBF-04 · Version 1.0

A Systematic Approach to Urine Sediment

A working method for scanning, identifying, quantifying, and reporting urine sediment so the result reflects the whole preparation, not just the most obvious field. Covers preparation and concentration factors, the fixed low-power to high-power scanning sequence, optical modes, automated particle analysis with manual confirmation, and documentation of specimen age and difficult identifications.

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Who this module is for

MLS/MLT students transitioning to bench practice and new bench technologists performing manual urine microscopy, after UBF-01 and microscope fundamentals.

Learning objectives

  • Prepare a representative sediment and explain how aliquot, residual volume, and loading affect concentration
  • Use a fixed scanning sequence and choose appropriate illumination and focus for low- and high-power examination
  • Quantify and report formed elements using the laboratory's own method and recognize when a result requires further review

How completion works

Mark every required section done and answer every knowledge check in those sections correctly. The final save completes the module automatically.

Sources

11 sources

  1. 1. 42 CFR § 493.1253, Establishment and verification of performance specifications. https://www.ecfr.gov/current/title-42/chapter-IV/subchapter-G/part-493/subpart-K/section-493.1253.

    Source note · federal regulation

  2. 2. 42 CFR § 493.1256, Standard: Control procedures. https://www.ecfr.gov/current/title-42/chapter-IV/subchapter-G/part-493/subpart-K/section-493.1256.

    Source note · federal regulation

  3. 3. 42 CFR § 493.1235, Standard: Personnel competency assessment policies. https://www.ecfr.gov/current/title-42/chapter-IV/subchapter-G/part-493/subpart-J/section-493.1235.

    Source note · federal regulation

  4. 4. Centers for Medicare & Medicaid Services. State Operations Manual, Appendix C: Survey Procedures and Interpretive Guidelines for Laboratories and Laboratory Services, interpretive guidance for §493.1256(d). https://www.cms.gov/regulations-and-guidance/guidance/manuals/downloads/som107ap_c_lab.pdf.

    Source note · federal guidance

  5. 5. College of American Pathologists. Urinalysis Checklist, URN.30800 Morphologic Observation Evaluation (Phase II). CAP Accreditation Program, 12.09.2025 edition.

    Source note · accreditation standard

  6. 6. Clinical and Laboratory Standards Institute. PRE05, Processes for the Collection of Urine Specimens, 1st ed. Consensus standard, 2024. https://clsi.org/shop/standards/pre05/.

    Source note · consensus standard

  7. 7. Clinical and Laboratory Standards Institute. H56-A, Body Fluid Analysis for Cellular Composition, 1st ed. Consensus standard, 2006, archived and technically valid per CLSI. https://clsi.org/shop/standards/h56/.

    Source note · consensus standard

  8. 8. Bujak A, et al. Preanalytics of urine sediment examination: effect of relative centrifugal force, tube type, volume of sample and supernatant removal. Biochem Med (Zagreb). 2018;28(1):010707. doi:10.11613/BM.2018.010707. https://pmc.ncbi.nlm.nih.gov/articles/PMC5806615/.

    Source note · peer-reviewed literature

  9. 9. Delanghe J, Speeckaert M. Preanalytical requirements of urinalysis. Biochem Med (Zagreb). 2014;24(1):89-104. doi:10.11613/BM.2014.011. https://pmc.ncbi.nlm.nih.gov/articles/PMC3936984/.

    Source note · peer-reviewed literature

  10. 10. Velez JCQ, et al. Diagnostic Utility of Urine Microscopy in Kidney Diseases. Kidney360. 2024. https://pmc.ncbi.nlm.nih.gov/articles/PMC11303840/.

    Source note · peer-reviewed literature

  11. 11. Siemens Healthineers. Atellica UAS 800 Analyzer Sample Processing Job Aid. https://academy.siemens-healthineers.com/_/en-us/atellica-uas-800-analyzer-sample-processing-job-aid/.

    Source note · manufacturer labeling