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Section 4 of 6 · Open sections

Required section · Section 4 of 6

Guided case: reading the worksheet

A clean-catch urine specimen arrives at the bench 90 minutes after collection, at ambient temperature. The worksheet documents the laboratory's own validated 10.0 mL aliquot, its validated centrifugation and resuspension steps, and a standardized coverslip preparation; these preparation values are not a procedure to copy.

The technologist follows the fixed sequence: a full low-power scan of the coverslip first. Clarity is recorded as slightly hazy. No crystals are seen on the initial bright-field low-power scan, and hyaline casts are noted at 0 to 1 per low-power field, within the laboratory's category for that finding. The scan is even; nothing suggests a poorly mixed or unevenly loaded preparation.

Moving to high power in several representative fields, squamous epithelial cells are rare, leukocytes are 0 to 2 per high-power field against a local adult interval of 0 to 5 per high-power field, and erythrocytes are 12 to 18 per high-power field against a local adult interval of 0 to 2 per high-power field. The dipstick panel run in parallel shows blood at 2+ and leukocyte esterase negative; the analyzer was not used for this case.

The erythrocyte count is reproducible across the representative fields examined, so the finding is that erythrocytes were observed above this worksheet's stated interval. That observation does not by itself establish where the blood originated or why. The blood pad's heme activity and the sediment erythrocyte count are distinct observations that agree in this case: the pad detects heme activity, while microscopy counts intact erythrocytes. The negative leukocyte esterase result and low leukocyte count also agree, but neither pair substitutes for the other or establishes cause.

Quantify what a systematic scan actually recovered, state the finding in the laboratory's own units and interval, and keep the sediment observation separate from any statement about cause.

Illustrative drawing — this picture was drawn rather than captured.

Chart with three columns, erythrocytes, leukocytes, and hyaline casts, each showing a light blue reference interval band, a teal upper-limit line, and the case result as a point; the erythrocyte result at 12 to 18 per high-power field is marked coral because it sits above the interval, the other two results sit within their bands.
Figure 1Guided case worksheet values for erythrocytes, leukocytes, and hyaline casts against the local reference interval.
Guided case worksheet, manual bright-field examination.
ItemResultLocal worksheet reference or flag
ClaritySlightly hazyDescriptive only
Dipstick blood2+Local strip instructions decision limit
Dipstick leukocyte esteraseNegativeLocal strip instructions decision limit
Erythrocytes, high power12 to 18 /HPFLocal adult interval 0 to 2 /HPF
Leukocytes, high power0 to 2 /HPFLocal adult interval 0 to 5 /HPF
Squamous epithelial cellsRareLocal category only
Hyaline casts, low power0 to 1 /LPFLocal category only
CrystalsNone seenNo reportable interval

Knowledge checks

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Knowledge check 1

In the guided case, the erythrocyte count of 12 to 18 per high-power field, against a local interval of 0 to 2 per high-power field, was reproducible across an even, representative scan. What can this finding support on its own?

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Knowledge check 2

The guided case shows dipstick blood at 2+ with a sediment erythrocyte count above interval, and dipstick leukocyte esterase negative with a leukocyte count within interval. How should these paired results be read?

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