Blood banking

Steps

1. 1Donors, collection and donor reactions16 min · Accept or defer a donor from history, medications, physical findings, and deferral records · Calculate a reduced collection volume and its matching anticoagulant volume · Distinguish vasovagal and citrate-related donor reaction patterns · Name the eligibility and testing rules that differ for directed and autologous donations
2. 2Releasing and storing components16 min · Verify donor testing and traceability before releasing a component · Store and transport each component at its labeled temperature and agitation · Determine component expiration after processing or system entry · Calculate red-cell processing recovery from volume and hematocrit
3. 3The red cell storage lesion14 min · Explain acidification and ATP changes during refrigerated red-cell storage · Predict the supernatant potassium change in refrigerated red cells · Match red-cell glycolysis and the pentose phosphate pathway to what each supplies
4. 4ABO, H and secretor status16 min · List the possible genotypes behind a blood-group phenotype in a person or family · Predict red-cell A and B antigens from H substance and the inherited transferase · Determine secretor status from ABH substances in saliva and match it to Lewis type · Recognize the Bombay phenotype and choose Bombay red cells for its anti-H
5. 5ABO subgroups and discrepancies17 min · Use reverse grouping, lectin tests, and history to explain a weak ABO forward reaction · Assign an adult ABO group from concordant forward and reverse results · Choose the next check for a discrepant ABO reaction pattern
6. 6D variants and the other blood group systems14 min · Choose the follow-up for a weak or discrepant D typing result · Judge whether a non-ABO antibody is significant from its thermal range and reactive phase
7. 7Reaction strength, complement and the antiglobulin test16 min · Predict how method, enhancement medium, and cell concentration change a reaction grade · Tell complement coating from proven hemolysis on a C3d-positive DAT · Tell in vivo coating (DAT) from in vitro coating (IAT) and what each reagent detects · Reject a negative tube antiglobulin test when required control cells fail
8. 8The antibody screen14 min · Explain why group O reagent cells are used for unexpected-antibody screening · Name the antibodies a negative screen can miss and the checks that still apply · Accept or reject a crossmatch sample by its age and the transfusion and pregnancy history
9. 9Identifying one antibody18 min · Use antigen-positive nonreactive cells for valid antibody exclusions
10. 10Two antibodies, the autocontrol and enzymes18 min · Tell an autoantibody from a new alloantibody by the autocontrol, DAT, and eluate · Read an enzyme-treated panel beside the untreated panel
11. 11Antibody history and antigen-negative units16 min · Select red cells using current and historical significant antibodies · Estimate how many units to screen from antigen-negative donor frequencies
12. 12Adsorption, elution, thiols and mixed fields18 min · Choose autologous or allogeneic adsorption from the recent transfusion history · Interpret an eluate against its last-wash control · Read DTT-treated test results knowing which antigens DTT destroys · Choose molecular follow-up when serologic phenotyping is unreliable
13. 13Crossmatching and issuing blood16 min · Choose an immediate-spin or antiglobulin crossmatch from the patient's antibody history · Determine eligibility for the validated electronic-crossmatch pathway · Verify a component at issue and state any testing still incomplete · Read column and solid-phase results using platform-specific endpoints
14. 14Hemolytic disease of the fetus and newborn17 min · Explain how maternal IgG and newborn bilirubin clearance produce HDFN findings · Recognize suppression of erythropoiesis in anti-K-associated fetal anemia · Call a maternal titer change only when the same method retests the earlier sample
15. 15Rh immune globulin and fetomaternal hemorrhage16 min · Determine postpartum Rh immune globulin eligibility from the laboratory results · Calculate fetomaternal hemorrhage volume from a Kleihauer-Betke count · Calculate the RhIG dose in whole syringes from the hemorrhage volume and product label
16. 16Cold autoantibodies and the Donath-Landsteiner test15 min · Judge cold-antibody significance from thermal amplitude and hemolysis · Interpret a Donath-Landsteiner test using its temperature sequence and controls
17. 17Warm and drug-induced immune hemolysis17 min · Exclude underlying alloantibodies and select antigen-matched units for a warm autoantibody · Tell drug-induced immune hemolysis from a delayed hemolytic reaction after transfusion
18. 18Choosing and modifying components16 min · Match cryoprecipitate, plasma, or another component to the factors that need replacing · Select plasma without donor antibodies against the recipient's red cells · Choose leukoreduction, irradiation, or washing for the risk each one reduces · Check a red-cell request against the hemoglobin threshold for the patient's setting
19. 19Transfusion reactions16 min · Perform the laboratory checks for a suspected transfusion reaction · Tell TRALI from circulatory overload by blood pressure, BNP, and fluid balance · Distinguish delayed serologic change from delayed hemolytic injury · Investigate suspected bacterial contamination of a transfused component
20. 20Look-back, interfaces and bedside safeguards15 min · Trace implicated components after a transfusion-transmitted infection report · Stop release when an interface converts a blank or invalid screen to negative · State the bedside identity check and administration rules for a component · Decide crossmatch or type and screen for a surgery from the blood order schedule
21. 21Platelet refractoriness, FNAIT and neutrophil antigens16 min · Calculate a corrected count increment with the stated platelet-dose units · Choose the next investigation for repeated poor platelet increments · Match maternal platelet antibodies to paternal platelet antigens in suspected FNAIT · Match HNA antibodies to HNA antigens in neonatal neutropenia and TRALI workups
22. 22Stem cell products and apheresis16 min · Calculate a viable CD34-positive cell dose per kilogram of recipient weight · Recalculate the viable-cell dose after each progenitor cell processing step · Choose red-cell and plasma groups after a major or minor ABO-mismatched transplant · Match an apheresis procedure to what it removes and what replaces it

What you'll be able to do