Blood Banking
Follow blood from donor to patient through component checks, blood group testing, antibody and compatibility testing, and hemolysis and transfusion reaction workups.
Steps
- Start
4 skills
- Accept or defer a donor from history, medications, physical findings, and deferral records
- Calculate a reduced collection volume and its matching anticoagulant volume
- Distinguish vasovagal and citrate-related donor reaction patterns
- Name the eligibility and testing rules that differ for directed and autologous donations
- Start
4 skills
- Verify donor testing and traceability before releasing a component
- Store and transport each component at its labeled temperature and agitation
- Determine component expiration after processing or system entry
- Calculate red-cell processing recovery from volume and hematocrit
At the bench Check component identity, storage, and suitability before issue
- Start
3 skills
- Explain acidification and ATP changes during refrigerated red-cell storage
- Predict the supernatant potassium change in refrigerated red cells
- Match red-cell glycolysis and the pentose phosphate pathway to what each supplies
- Start
4 skills
- List the possible genotypes behind a blood-group phenotype in a person or family
- Predict red-cell A and B antigens from H substance and the inherited transferase
- Determine secretor status from ABH substances in saliva and match it to Lewis type
- Recognize the Bombay phenotype and choose Bombay red cells for its anti-H
At the bench Resolve a blood group discrepancy before selecting compatible components
- Start
3 skills
- Use reverse grouping, lectin tests, and history to explain a weak ABO forward reaction
- Assign an adult ABO group from concordant forward and reverse results
- Choose the next check for a discrepant ABO reaction pattern
At the bench Resolve a blood group discrepancy before selecting compatible components
- Start
2 skills
- Choose the follow-up for a weak or discrepant D typing result
- Judge whether a non-ABO antibody is significant from its thermal range and reactive phase
At the bench Resolve a blood group discrepancy before selecting compatible components · Use the antibody workup to select suitable donor units
- Start
4 skills
- Predict how method, enhancement medium, and cell concentration change a reaction grade
- Tell complement coating from proven hemolysis on a C3d-positive DAT
- Tell in vivo coating (DAT) from in vitro coating (IAT) and what each reagent detects
- Reject a negative tube antiglobulin test when required control cells fail
At the bench Use the antibody workup to select suitable donor units · Perform a direct antiglobulin workup and interpret its controls
- StartThe antibody screen14 min
3 skills
- Explain why group O reagent cells are used for unexpected-antibody screening
- Name the antibodies a negative screen can miss and the checks that still apply
- Accept or reject a crossmatch sample by its age and the transfusion and pregnancy history
At the bench Use the antibody workup to select suitable donor units · Check specimen identity and suitability before analysis
- StartIdentifying one antibody18 min
1 skill
- Use antigen-positive nonreactive cells for valid antibody exclusions
At the bench Use the antibody workup to select suitable donor units
- Start
2 skills
- Tell an autoantibody from a new alloantibody by the autocontrol, DAT, and eluate
- Read an enzyme-treated panel beside the untreated panel
At the bench Use the antibody workup to select suitable donor units
- Start
2 skills
- Select red cells using current and historical significant antibodies
- Estimate how many units to screen from antigen-negative donor frequencies
At the bench Use the antibody workup to select suitable donor units
- Start
4 skills
- Choose autologous or allogeneic adsorption from the recent transfusion history
- Interpret an eluate against its last-wash control
- Read DTT-treated test results knowing which antigens DTT destroys
- Choose molecular follow-up when serologic phenotyping is unreliable
At the bench Use the antibody workup to select suitable donor units
- Start
4 skills
- Choose an immediate-spin or antiglobulin crossmatch from the patient's antibody history
- Determine eligibility for the validated electronic-crossmatch pathway
- Verify a component at issue and state any testing still incomplete
- Read column and solid-phase results using platform-specific endpoints
At the bench Check component identity, storage, and suitability before issue · Use the antibody workup to select suitable donor units
- Start
3 skills
- Explain how maternal IgG and newborn bilirubin clearance produce HDFN findings
- Recognize suppression of erythropoiesis in anti-K-associated fetal anemia
- Call a maternal titer change only when the same method retests the earlier sample
- Start
3 skills
- Determine postpartum Rh immune globulin eligibility from the laboratory results
- Calculate fetomaternal hemorrhage volume from a Kleihauer-Betke count
- Calculate the RhIG dose in whole syringes from the hemorrhage volume and product label
- Start
2 skills
- Judge cold-antibody significance from thermal amplitude and hemolysis
- Interpret a Donath-Landsteiner test using its temperature sequence and controls
At the bench Use the antibody workup to select suitable donor units
- Start
2 skills
- Exclude underlying alloantibodies and select antigen-matched units for a warm autoantibody
- Tell drug-induced immune hemolysis from a delayed hemolytic reaction after transfusion
At the bench Use the antibody workup to select suitable donor units · Perform and document the laboratory workup of a transfusion reaction
- Start
4 skills
- Match cryoprecipitate, plasma, or another component to the factors that need replacing
- Select plasma without donor antibodies against the recipient's red cells
- Choose leukoreduction, irradiation, or washing for the risk each one reduces
- Check a red-cell request against the hemoglobin threshold for the patient's setting
At the bench Check component identity, storage, and suitability before issue
- StartTransfusion reactions16 min
4 skills
- Perform the laboratory checks for a suspected transfusion reaction
- Tell TRALI from circulatory overload by blood pressure, BNP, and fluid balance
- Distinguish delayed serologic change from delayed hemolytic injury
- Investigate suspected bacterial contamination of a transfused component
At the bench Perform and document the laboratory workup of a transfusion reaction
- Start
4 skills
- Trace implicated components after a transfusion-transmitted infection report
- Stop release when an interface converts a blank or invalid screen to negative
- State the bedside identity check and administration rules for a component
- Decide crossmatch or type and screen for a surgery from the blood order schedule
At the bench Perform and document the laboratory workup of a transfusion reaction
- Start
4 skills
- Calculate a corrected count increment with the stated platelet-dose units
- Choose the next investigation for repeated poor platelet increments
- Match maternal platelet antibodies to paternal platelet antigens in suspected FNAIT
- Match HNA antibodies to HNA antigens in neonatal neutropenia and TRALI workups
At the bench Check component identity, storage, and suitability before issue
- Start
4 skills
- Calculate a viable CD34-positive cell dose per kilogram of recipient weight
- Recalculate the viable-cell dose after each progenitor cell processing step
- Choose red-cell and plasma groups after a major or minor ABO-mismatched transplant
- Match an apheresis procedure to what it removes and what replaces it
At the bench Check component identity, storage, and suitability before issue